| 1. | Sandwich elisa assay is a sensitive , specific and stable technique
巩乃a法检测可溶性比卜工灵敏、特异、稳定； 2 |
| 2. | And further studied e rosettes experiment and lymphocyte proliferation brdu - elisa assay
并对初步筛选的e玫瑰花环实验和淋巴细胞增殖brdu - elisa实验进行了进一步筛选。 |
| 3. | Finally , we ensure immunobiologic activity evaluation methods of gpif wre t cells from pigs thymus of e rosettes experiment and pha inducing lymphocyte proliferation brdu - elisa assay
最终确定gpif免疫生物活性评价方法为： t细胞来源于胸腺的e玫瑰花环实验和pha诱导的淋巴细胞增殖brdu - elisa实验。 |
| 4. | 5 . the mpcr - rflp assay was useful for its reliability in monitoring hbv ymdd mutants . melting curve assay and pcr microplate hybridization - elisa assay should be further improved to increase their sensitivity and specialty
5 . mpcr一rflp法检测ymdd突变株具有较好的可靠性和可行性，是监测拉米夫定耐药株的一种非常有效的方法;熔解曲线法和pcr微板核酸杂交法需要进一步完善以提高敏感性和特异性。 |
| 5. | Western blot and elisa assays indicated that fmdv vp1 gene was expressed in tobacco chloroplasts and accounted for 2 % ~ 3 % of the total soluble protein ( about 20 - 30 ( ig of chloroplast - expressed vp1 protein in 1 mg of total soluble protein )
Westernblot和elisa免疫杂交分析证明fmdvvp1抗原蛋白在烟草叶绿体中得到表达，表达量占可溶性总蛋白量的2 3 ( 1mg可溶性总蛋白含有20 30 g的vp1蛋白) 。 |
| 6. | [ methods ] consideration of free heparin , unlike polypeptides , adsorb poorly onto plastic surfaces . in order to study the interaction of heparin with ifn - y using elisa assay , heparin - bovine complex ( hbc ) was synthesized by conjugating heparin to bsa in the presence of sodium cyanoborohydride
结果凝胶过滤层析获得了高纯度的hbc ，通过hbc将肝素包被在酶标板上， ellsa实验表明， ifn一y主要与hbc中的肝素结合( p ( o ， 01 ) 。 |
| 7. | Methods : sandwich elisa assay was used , w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody . the second antibody - hrp conjugate was added for coloration . standard curve was obtained by shla - i standard reagent in serial dilution . the amount of shla - i in the samples was determined : 1
方法：以w6 32包被酶标板，捕捉样品中可溶性hla ，加入一抗2m抗体，再加酶标二抗及底物显色。根据可溶性hla -的不同浓度标准品显色后的od值绘制标准曲线： 1 |
| 8. | 3 . the choosing of optimum evaluating methods of immunobiologic activity : if we disposed sheep blood red cells with neuraminic acid enzyme , the e rosettes forming ratio remarkbly increased ; we improved the conventional method of brdu - elisa assay on lymphocyte proliferation induced by cona . the results showed that lymphocyte proliferation induced by pha was better than by cona . 4
3 、 gpif免疫生物活性评价方法的优化对gpife玫瑰花环实验在不影响srbc活性的基础上用神经氨酸酶处理，经此法处理后e玫瑰花环形成率明显提高；对传统的cona诱导的人外周血淋巴细胞增殖brdu - elisa实验，进行了方法学改进。 |
| 9. | The expression activity reached the highest point at the 72nd hour in bmn cell ( 22 u / 2x ! 06cells ) and at 144th hour in larvae ( 159 u / ml ) , respectively . elisa assay showed that expression product had angiostatin ' s immunoreactivity . western blotting assay also showed that product expressed in cultured cells was a 36 kd band , while product expressed in larvae of silkworms was two proteins , and the molecular weights were 37 kd and 42 kd , respectively
表达产物活性在家蚕细胞中表达72小时达到最高值，经胞内表达产物作用后，血管内皮细胞的存活率仅为( 28 . 0 3 . 0 ) ，表达量约为22u 2 10 ~ 6个细胞；在家蚕体内表达144小时活性达到最高值，内皮细胞的存活率仅为( 6 . 4 0 . 5 ) ，表达量约159u ml 。 |
